determination of self-compatible almond genotypes obtained from controlled crosses and identification of s-alleles in some foreign cultivars and genotypes by pcr

Authors

علی عبادی

a. ebadi university college of agriculture and natural resources, university of tehran,پردیس کشاورزی و منابع طبیعی دانشگاه تهران کاظم کمالی

k. kamali پردیس کشاورز ی و منابع طبیعی دانشگاه تهران محمدرضا فتاحی مقدم

m. r. fattahi moghaddam پردیس کشاورزی و منابع طبیعی دانشگاه تهران محمدرضا نقوی

m. r. naghavi علی ایمانی

abstract

self-incompatibility is one of the main problems in almond production. in this research work, at the first step, some local cultivars as well as some imported ones were evaluated from the point of their male and female organ compatibility. then, ten selected commercial self-incompatible cultivars(female parents) were crossed with two self-compatible cultivars(male parents) i. e. tuono and supernova. to discriminate self-compatible progenies from others, all seedlings were evaluated through bagging method and molecular marker. with using sff-sfr primers, self-compatible genotypes produced band of 450bp. however, self-incompatible genotypes did not produce any band. according to the results, out of 195 seedlings, 110 were self-compatible and the other 85 were identified as self-incompatible. this ratio was according to the mendelian law and there was no significant difference between the number of cultivars or genotypes in two groups. in another work, s-alleles of some doubtful cultivars and genotypes as well as their situation from the point of sf were determined by simple and multiplex pcr.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Identification of Self- incompatibility Alleles in Some Almond Genotypes by Degenerate S-RNase Primers

The almond, Prunus dulcis Miller which belongs to Rosaceae family, is one of the most important commercial and oldest cultivated tree nut crops. Almonds are classified as a ‘nut’ in which the edible seed is the commercial product. Therefore, pollination and fertilization are necessary in almond. The characteristic of cultivated almond to express gametophytic self- incompatibility discourages se...

full text

The Study of Morphological Traits and Identification of Self-incompatibility Alleles in Almond Cultivars and Genotypes

The evaluation of an almond collection using morphological variables and identification of self-incompatibility genotype  is useful for selecting pollinizers and for the design of crossing in almond breeding programs. In this study, important morphological traits and self-incompatibilities in 71 almond cultivars and genotypes were studied. Simple and multiplex specific PCR analyses were used in...

full text

identification of self-incompatibility (s-) genotypes of iranian almond genotypes and cultivars using pcr

almond (prunus dulcis [webb] d.a. mill) is an economically important species of genus prunus (rosaceae, subfamily prunoideae). the commercial edible part of almond fruit is kernel. fertilization is essential for nut production in almond but most cultivars are self or even cross-incompatible. self-incompatibility in almond is gametophytic and controlled by a single s-locus with multiple codomina...

full text

Study of Compatibility Relationships Among Some Almond Cultivars and Genotypes Using of SAlleles Identification

Almond (Prunus dulcis L.) is one of the most important nut crops in Iran. Most almond cultivars and genotypes are self-incompatible. However, research on S-alleles indicates that it is very efficient in cultivar selection. Selfincompatibility in almond is gametophytic and controlled by a single S-locus with multiple codominant alleles. In this study, compatibility relationships among cultivars,...

full text

My Resources

Save resource for easier access later


Journal title:
به نژادی نهال و بذر

جلد ۲۷، شماره ۱، صفحات ۵۷-۶۷

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023